Shifts in microbial community composition can occur because of local or site-to-site variations or system-scale changes that affect the whole distribution system. Intentional disturbances from drinking water treatment facility operations improve water quality in the long-term, but can have unintended consequences. The goal of this project is to investigate the effect of disturbances on microbial communities in distribution systems and inform management practices.
Kennedy Water Lab
Applying principles of microbiology, data science, and statistics to protect public health and the environment
We aim to characterize microbial community dynamics, occurrence, and persistence in the engineered water cycle and apply these data to protect public health and wellbeing as well as the environment.
Research
Effects of disturbances on microbial community dynamics in distribution systems.
Wastewater-based epidemiology (WBE) for potable reuse
Advanced-treated wastewater can be used as a source of drinking water through direct potable reuse. WBE can help ensure ingestion of recycled water does not result in increased enteric diseases in the contributing community. The goal of this project is to quantify and compare pathogen occurrence in the engineered water cycle for communities with direct, indirect, and de facto potable reuse, and develop a inform monitoring strategies for potable reuse.
Mechanisms of virus inactivation
Enteric viruses in the engineered water cycle are generally quantified via culture methods or molecular methods. While culture methods are ideal for quantifying infectious viruses, molecular methods are still commonly used because they tend to have higher sensitivity and faster turnaround times and not all viruses are easily culturable. The goal of this project is to develop and evaluate molecular methods to assess enteric virus inactivation mechanisms and persistence in the engineered water cycle.
Enhanced microbial monitoring in the engineered water cycle
Enhanced methods of microbial assessment include methods for microbial abundance (e.g., flow cytometry-based cell counts), microbial community composition (e.g., DNA sequencing), and specific microbial targets (e.g., qPCR). The goal of this project is to combine high resolution enhanced microbial assessment data along with physicochemical water quality data and machine learning could aid in early identification of common issues in water systems.
Group Members
Lauren C Kennedy
Principal Investigator
Dr. Kennedy is an assistant professor in the department of Civil Engineering at the University of Texas at El Paso. She earned her B.S. in Environmental and Ecological Engineering at Purdue University and her M.S. as well as her Ph.D. in Civil and Environmental Engineering from the University of California, Berkeley. She continued her training as a postdoc at Stanford University, where she joined the Sewer Coronavirus Alert Network (SCAN) and wastewaterSCAN teams. The Kennedy Water Lab aims to characterize microbial community dynamics, occurrence, and persistence in the engineered water cycle and apply these data to protect public health and wellbeing as well as the environment.
Mya Valenzuela
PhD Student
I am currently a 1st year PhD student researcher in Civil/Environmental Engineering focusing based epidemiology (WBE). I am passionate about my work and making my community a more sustainable place to live. Outside of my research I enjoy staying active outdoors, and traveling!
Angelica Littles
Undergraduate Student
Angelica is an undergraduate Civil Engineering student with a minor in Mathematics. Her current research focuses on managing opportunistic pathogens in drinking water distribution systems. Before joining the lab, Angelica participated in the Louis Stokes Alliance for Minority Participation (LSAMP) Program, where she spent a summer at the University of Texas at San Antonio researching permeable curbs for stormwater pollution. In addition, she wrote a conference paper for the American Society of Engineering Education (ASEE) where she explored her experience as a multiracial individual attending a Hispanic Serving Institute. Aside from research, Angelica is active in multiple student organizations on campus. Angelica was an officer for Chi Epsilon, helped restart the UTEP National Society of Black Engineers (NSBE) chapter, and currently competes with the UTEP forensics speech and debate team. Outside of school, Angelica enjoys reading books, writing stories, baking, traveling, crocheting, embroidery, scrapbooking, trying new restaurants, and watching period dramas.
Cristian Mireles
Undergraduate Student
Cristian is a senior in the Civil Engineering program at UTEP looking to specialize in the water treatment pathway. Currently he is working as an undergraduate research assistant on the UV/AOP project. He is especially passionate about the treatment and consumption of water and its current political implications as they pertain to the greater El Paso area due to having grown up in the region and being exposed to the realities water scarcity and thus hopes to make contribution toward the progress solving this issue. To add he is currently the president of the ASCE student chapter at UTEP which is one of the oldest and most storied organizations on campus but is most known for its success in the annual Concrete Canoe competition which requires the full-scale design and construction of canoe made of specially made concrete. On his free time, he enjoys hiking with his dog and training to have an amateur boxing debut.
Stephanie Romero
Undergraduate Student
Stephanie Romero is an undergraduate student majoring in Civil Engineering, with an associate degree in Architecture. Her current research focuses on analyzing the effects of drinking water source shifts using flow cytometry. After her studies, Stephanie plans to specialize in Water and Environmental Engineering. She is actively engaged as the President of the student chapter of the National Society of Professional Engineers (NSPE). In her free time, Stephanie enjoys drawing, crocheting, gaming, listening to music, and writing.
Publications
Publications last scraped from Google Scholar for Lauren C Kennedy in January 2024
*authors contributed equally to the work
Temperature and particles interact to affect human norovirus and MS2 persistence in surface water
LC Kennedy, SA Lowry, AB Boehm
(2024). Environmental Science: Processes & Impacts
Identifying trends in SARS-CoV-2 RNA in wastewater to infer changing COVID-19 incidence: Effect of sampling frequency
EMG Chan, LC Kennedy, MK Wolfe, AB Boehm
(2023). PLOS Water
Persistence of Human Norovirus (GII) in Surface Water: Decay Rate Constants and Inactivation Mechanisms
LC Kennedy, VP Costantini, KA Huynh, SK Loeb, WC Jennings, S Lowry, ...
(2023). Environmental Science & Technology 57 (9), 3671-3679
Stay in the loop: lessons learned about the microbial water quality in pipe loops transitioned from conventional to direct potable reuse water
LC Kennedy*, SE Miller*, RS Kantor, HD Greenwald, MJ Adelman, ...
(2023). Environmental Science: Water Research & Technology
Microbial Water Quality through a Full-Scale Advanced Wastewater Treatment Demonstration Facility
S Miller, H Greenwald, LC Kennedy, RS Kantor, R Jiang, A Pisarenko, ...
(2022). ACS Es&t Engineering 2 (12), 2206-2219
Is flushing necessary during building closures? A study of water quality and bacterial communities during extended reductions in building occupancy
H Greenwald, LC Kennedy, A Ehde, Y Duan, CI Olivares, R Kantor, ...
(2022). Frontiers in Water 4, 958523
Estimating Relative Abundance of 2 SARS-CoV-2 Variants through Wastewater Surveillance at 2 Large Metropolitan Sites, United States
AT Yu, B Hughes, MK Wolfe, T Leon, D Duong, A Rabe, LC Kennedy, ...
(2022). Emerging Infectious Diseases 28 (5), 940-947
Operationalizing a routine wastewater monitoring laboratory for SARS-CoV-2
RS Kantor, HD Greenwald, LC Kennedy, A Hinkle, S Harris-Lovett, ...
(2022). PLOS Water 1 (2), e0000007
Legionella pneumophila occurrence in reduced-occupancy buildings in 11 cities during the COVID-19 pandemic (preprint)
K Dowdell, H Greenwald, S Joshi, M Grimard-Conea, S Pitell, Y Song, ...
(2022).
Comparison of RT-qPCR and digital PCR methods for wastewater-based testing of SARS-CoV-2
A Hinkle, HD Greenwald, M Metzger, M Thornton, LC Kennedy, K Loomis, ...
(2022). medRxiv, 2022.06. 15.22276459
SARS-CoV-2 RNA is enriched by orders of magnitude in primary settled solids relative to liquid wastewater at publicly owned treatment works
S Kim, LC Kennedy, MK Wolfe, CS Criddle, DH Duong, A Topol, BJ White, ...
(2022). Environmental Science: Water Research & Technology 8 (4), 757-770
Tools for interpretation of wastewater SARS-CoV-2 temporal and spatial trends demonstrated with data collected in the San Francisco Bay Area
HD Greenwald*, LC Kennedy*, A Hinkle, ON Whitney, VB Fan, ...
(2021). Water Research X 12, 100111
Sewage, Salt, Silica, and SARS-CoV-2 (4S): An Economical Kit-Free Method for Direct Capture of SARS-CoV-2 RNA from Wastewater
ON Whitney, LC Kennedy, VB Fan, A Hinkle, R Kantor, H Greenwald, ...
(2021). Environmental science & technology 55 (8), 4880-4888
Challenges in Measuring the Recovery of SARS-CoV-2 from Wastewater
RS Kantor, KL Nelson, HD Greenwald, LC Kennedy
(2021). Environmental Science & Technology 55 (6), 3514-3519
Genome sequencing of sewage detects regionally prevalent SARS-CoV-2 variants
A Crits-Christoph, RS Kantor, MR Olm, ON Whitney, B Al-Shayeb, YC Lou, ...
(2021). MBio 12 (1), e02703-20
Effect of disinfectant residual, pH, and temperature on microbial abundance in disinfected drinking water distribution systems
LC Kennedy, SE Miller, RS Kantor, KL Nelson
(2021). Environmental Science: Water Research & Technology 7 (1), 78-92
High-throughput DNA sequencing to profile microbial water quality of potable reuse
MB Leddy, MH Plumlee, RS Kantor, KL Nelson, SE Miller, LC Kennedy, ...
(2018). Water Innov, 33-37
CNC-loaded hydrogel particles generated from single-and double-emulsion drops
C Ye, L Kennedy, K Shirk, UM Córdova-Figueroa, J Youngblood, ...
(2015). Green Materials 3 (1), 25-34
Teaching
Join
If you are interested in joining the Kennedy Water Lab, reach out to Dr. Kennedy with the folowing:
Please contact Dr. Kennedy if you have any questions.